Plasmid

Part:BBa_K3819023

Designed by: Luo SongWen   Group: iGEM21_UESTC-China   (2021-09-28)


Lipase-Balac plasmid

We put the laccase Blac gene on a plasmid together with the lipase gene for the reason that these enzymes both work in the form of the free enzyme instead of binding to the dockerin domain. The genes for lipase and laccase are constructed on the same plasmid, and they are connected using 2A peptide. In the 2A peptide, we chose E2A, which can cut the Balac and lipase after they are expressed, so that the two enzymes can perform their respective functions.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 727
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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